Coding

Part:BBa_K1333111

Designed by: Zhang Fangyingnan   Group: iGEM14_SYSU-China   (2014-10-17)

DNAQ CDS

a subunit of DNA polymeraseIII


Usage and Biology

DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication.

SOS error prone bypass

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutagenesis

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
    Illegal AgeI site found at 535
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None